Aliidiomarina shirensis as Possible Source of the Integron- and Plasmid-Mediated Fosfomycin Resistance Gene fosC2.

ABSTRACT: In-silico analysis and cloning experiments identified a fosC2-like fosfomycin resistance gene in the chromosome of Aliidiomarina shirensis, with our data suggesting that this bacterium might be added to the list of species identified as reservoirs of fos-like genes that were subsequently acquired by other Gram-negative species. Indeed, the fosC2 gene was identified as acquired in Providencia huaxinensis and Aeromonas hydrophila isolates, with this gene being located in class 1 integron structures in the latter cases. Biochemical characterization and site-directed mutagenesis showed a higher catalytic efficiency for the intrinsic FosC2^AS (from A. shirensis) than for the acquired FosC2 (from P. huaxinensis) enzyme due to a single substitut

SUBMITTER: Ortiz de la Rosa JM 

PROVIDER: S-EPMC8923222 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature