Discovery of a potent cytotoxic agent that promotes G₂/M phase cell cycle arrest and apoptosis in a malignant human pharyngeal squamous carcinoma cell line.

ABSTRACT: Squamous cell carcinoma is the major form of malignancy that arises in head and neck cancer. The modest improvement in the 5‑year survival rate underpins its complex etiology and provides the impetus for the discovery of new therapeutics. The present study describes the discovery of an indole‑based small molecule (24a) that was a potent cytotoxic agent with antiproliferative and pro‑apoptotic properties against a pharyngeal carcinoma cell line, Detroit 562, effectively killing the cells at a half‑maximal inhibitory concentration of 0.03 µM, as demonstrated using cell proliferation studies. The antiproliferative property of 24a was demonstrated by its ability to promote G₂/M blockade, as assessed by cell cycle analysis using flow cytometry and the monitoring of real‑time cell cyc

SUBMITTER: Nuth M 

PROVIDER: S-EPMC8923654 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature