G-protein coupled receptor 34 regulates the proliferation and growth of LS174T cells through differential expression of PI3K subunits and PTEN.

ABSTRACT:

Purpose

G-protein coupled receptor (GPR 34) has been found to play important roles in some cancers and regulates the proliferation, apoptosis, and migration of these cancer cells. However, the mechanisms underlying how GPR34 functions to regulate growth and proliferation of colorectal cancer cells remains to be clarified.

Methods

We employed stable GPR34 knockdown LS174T cell models, GPR34 Mab blocking, a CCK-8 kit, and a colony formation assay to characterize the effect of GPR34 on the proliferation of LS174T in vitro and xenograft tumor growth in vivo. The mRNA level of GPR34 was detected by RT-PCR in tumor tissues and adjacent normal tissues from 34 CRC patients.

Results

Based on RT-PCR results, GPR34 exhibited high level in tumor samples compared with adjacent nor

SUBMITTER: Zuo B 

PROVIDER: S-EPMC8924081 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature