Metabolic fingerprinting for discrimination of DNA-authenticated Atractylodes plants using ¹H NMR spectroscopy.

ABSTRACT: Identifying different species of the genus Atractylodes which are commonly used in Chinese and Japanese traditional medicine, using chromatographic approaches can be difficult. ¹H NMR metabolic profiling of DNA-authenticated, archived rhizomes of the genus Atractylodes was performed for genetic and chemical evaluation. The ITS region of the nuclear rDNA was sequenced for five species, A. japonica, A. macrocephala, A. lancea, A. chinensis, and A. koreana. Our samples had nucleotide sequences as previously reported, except that part of the A. lancea cultivated in Japan had a type 5, hybrid DNA sequence. Principal component analysis (PCA) using ¹H NMR spectra of extracts with two solvent systems (CD₃OD, CDCl₃) was performed. When CDCl₃ ex

SUBMITTER: Shirahata T 

PROVIDER: S-EPMC8924082 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature