The role of microRNA-155 in glomerular endothelial cell injury induced by high glucose.

ABSTRACT:

Objective

To investigate the role of microRNA-155-5p on apoptosis and inflammatory response in human renal glomerular endothelial cells (HRGEC) cultured with high glucose.

Methods

The primary HRGEC were mainly studied, light microscopy was used to detect changes in cell morphology. Quantitative Real Time-Polymerase Chain Reaction, Western Blot, immunofluorescence were aimed to observe the mRNA and protein expression levels of target gene ETS-1, downstream factors VCAM-1, MCP-1 and cleaved caspase-3 in each group after high glucose treatment as well as transfection with miR-155 mimics or inhibitor.

Results

The expression of inflammatory factors and apoptosis of HRGEC cells increased under high glucose treatment. Compared with normal-glucose treatment, the expression of

SUBMITTER: He K 

PROVIDER: S-EPMC8924107 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature