Hepatic stellate cell mediates transcription of TNFSF14 in hepatocellular carcinoma cells via H₂S/CSE-JNK/JunB signaling pathway.

ABSTRACT: Hepatic stellate cells (HSC) and hydrogen sulfide (H₂S) both play important roles in the development of hepatocellar carcinoma (HCC). Whereas, in the microenvironment of HCC, whether HSC participate in regulating the biological process of HCC cells by releasing H₂S remains elusive. In vitro, Flow cytometry (FCM), CCK-8, RNA-sequencing, Western blotting, RT-qPCR, immunofluorescence and ChIP assays were carried out in the HCC cells to investigate the effect of H₂S on biological functions and JNK/JunB-TNFSF14 signaling pathway. Specimens from HCC patients were analyzed by RT-qPCR and Western blotting assays for evaluating the expression of TNFSF14 and CSE. Statistical analysis was used to analyze the correlation between TNFSF14 expression and clinical data of

SUBMITTER: Ma Y 

PROVIDER: S-EPMC8924155 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature