Low Cost, Easily-Assembled Centrifugal Buoyancy-Based Emulsification and Digital PCR.

ABSTRACT: Microfluidic-based droplet generation approaches require the design of microfluidic chips and a precise lithography process, which require skilled technicians and a long manufacturing time. Here we developed a centrifugal buoyancy-based emulsification (CBbE) method for producing droplets with high efficiency and minimal fabrication time. Our approach is to fabricate a droplet generation module that can be easily assembled using syringe needles and PCR tubes. With this module and a common centrifuge, high-throughput droplet generation with controllable droplet size could be realized in a few minutes. Experiments showed that the droplet diameter depended mainly on centrifugal speed, and droplets with controllable diameter from 206 to 158 μm could be generated under a centrifugal acceleration

SUBMITTER: Zhou W 

PROVIDER: S-EPMC8924881 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature