Serum exosomal proteomics analysis of lung adenocarcinoma to discover new tumor markers.

ABSTRACT:

Background

Among the most aggressive and rapidly lethal types of lung cancer, lung adenocarcinoma is the most common type. Exosomes, as a hot area, play an influential role in cancer. By using proteomics analysis, we aimed to identify potential markers of lung adenocarcinoma in serum.

Methods

In our study, we used the ultracentrifugation method to isolate serum exosomes. The Liquid chromatography-mass spectrometry (LC-MS) and bioinformatics analysis were used to identify potential serum exosomal proteins with altered expression among patients with advanced lung adenocarcinoma, early lung adenocarcinoma, and healthy controls. A western blot (WB) was performed to confirm the above differential expression levels in a separate serum sample-isolated exosome, and immunohistochemis

SUBMITTER: Liu S 

PROVIDER: S-EPMC8925168 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature