Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the GDNF-LIF Interaction.

ABSTRACT:

Purpose

To investigate the role of the miR-211-5p-GDNF signaling pathway in carboplatin resistance of retinoblastoma Y79 cells and what factors it may be affected by.

Methods

A carboplatin-resistant retinoblastoma cell line (Y79R) was established in vitro. RNA-seq and microRNA-seq were constructed between Y79 and Y79R cells. RNA interference, RT-PCR, Western blot (WB), and flow cytometry were used to verify the expression of genes and proteins between the two cell lines. The TargetScan database was used to predict the microRNAs that regulate the target genes. STING sites and Co-Immunoprecipitation (COIP) were used to study protein-protein interactions.

Results

GDNF was speculated to be the top changed gene in the drug resistance in Y79R cell lines. Moreover, th

SUBMITTER: Ke N 

PROVIDER: S-EPMC8925320 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature