Periductal bile acid exposure causes cholangiocyte injury and fibrosis.

ABSTRACT:

Introduction

Bile duct integrity is essential for the maintenance of the structure and function of the biliary tree. We previously showed that cholangiocyte injury in a toxic model of biliary atresia leads to increased monolayer permeability. Increased epithelial permeability was also shown in other cholangiopathies. We hypothesized that after initial cholangiocyte injury, leakage of bile acids into the duct submucosa propagates cholangiocyte damage and fibrosis. We thus aimed to determine the impact of bile acid exposure on cholangiocytes and the potential therapeutic effect of a non-toxic bile acid.

Materials and methods

Extrahepatic bile duct explants were isolated from adult and neonatal BALB/c mice. Explants were cultured with or without glycochenodeoxycholic acid and u

SUBMITTER: Dotan M 

PROVIDER: S-EPMC8926245 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature