Transfer and Expression of Native Human Insulin-Like Growth Factor-1 in Tobacco Chloroplasts.

ABSTRACT:

Background

Insulin-like growth factor-1 (IGF-1), in addition to having insulin-like effects, has boosting effects on all cells in human body. Most of the recombinant IGF-1 required for patients suffering from its deficiency is currently produced by bacterial and yeast systems. Plant systems, especially chloroplasts, have many benefits for producing human blood proteins. Production costs are low in these systems, and their side effects are less than other systems.

Objectives

In this study, the transfer and expression of mature IGF-1 protein cDNA in tobacco chloroplasts under the control of strong plastid transcription and translation elements was evaluated.

Materials and methods

The biolistic transformation method was used to transfer the IGF-1 gene cloned into

SUBMITTER: Ahmadabadi M 

PROVIDER: S-EPMC8926313 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature