Effect of inactivated nature-derived microbial composition on mouse immune system.

ABSTRACT:

Introduction

The hygiene hypothesis suggests that decrease in early life infections due to increased societal-level hygiene standards subjects one to allergic and autoimmune diseases. In this report, we have studied the effect of sterilized forest soil and plant-based material on mouse immune system and gut microbiome.

Methods

Inbred C57Bl/6 mice maintained in normal sterile environment were subjected to autoclaved forest soil-derived powder in their bedding for 1 h a day for 3 weeks. Immune response was measured by immune cell flow cytometry, serum cytokine enzyme-linked immunoassay (ELISA) and quantitative polymerase chain reaction (qPCR) analysis. Furthermore, the mouse gut microbiome was analyzed by sequencing.

Results

When compared to control mice, mice treated w

SUBMITTER: Gonzalez-Rodriguez MI 

PROVIDER: S-EPMC8926502 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature