Single-cell RNA-seq reveals clonal diversity and prognostic genes of relapsed multiple myeloma.

ABSTRACT:

Background

Multiple myeloma (MM) is a clinically and biologically heterogeneous plasma-cell malignancy. Despite extensive research, disease heterogeneity and relapse remain a big challenge in MM therapeutics. We tried to dissect this disease and identify novel biomarkers for patient stratification and treatment outcome prediction by applying single-cell technology.

Methods

We performed single-cell RNA sequencing (scRNA-seq) and variable-diversity-joining regions-targeted sequencing (scVDJ-seq) concurrently on bone marrow samples from a cohort of 18 patients with newly diagnosed MM (NDMM; n = 12) or refractory/relapsed MM (RRMM; n = 6). We analysed the malignant clonotypes using scVDJ-seq data and conducted data integration and cell-type annotation through the CCA algorithm b

SUBMITTER: He H 

PROVIDER: S-EPMC8926895 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature