Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.

ABSTRACT: Engineering the microbial production of secondary metabolites is limited by the known reactions of correctly annotated enzymes. Therefore, the machine learning discovery of specialized enzymes offers great potential to expand the range of biosynthesis pathways. Benzylisoquinoline alkaloid production is a model example of metabolic engineering with potential to revolutionize the paradigm of sustainable biomanufacturing. Existing bacterial studies utilize a norlaudanosoline pathway, whereas plants contain a more stable norcoclaurine pathway, which is exploited in yeast. However, committed aromatic precursors are still produced using microbial enzymes that remain elusive in plants, and additional downstream missing links remain hidden within highly duplicated plant gene families. In the curre

SUBMITTER: Vavricka CJ 

PROVIDER: S-EPMC8927377 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature