Production of biologically active recombinant buffalo leukemia inhibitory factor (BuLIF) in Escherichia Coli.

ABSTRACT:

Background

Leukemia inhibitory factor (LIF) is a multifunctional cytokine which plays multiple roles in different biological processes such as implantation, bone remodeling, and hematopoiesis. The buESCs are difficult to culture due to lack of proper understanding of the culture conditions. LIF is one of the important factors which maintain the pluripotency in embryonic stem cells and commercial LIF from murine and human origin is used in the establishment of buffalo embryonic stem cells (buESCs). The LIF from a foreign origin is not able to maintain pluripotency and proliferation in buESCs for a long term which is contributed by difference in the binding sites on LIF; therefore, culture medium supplemented with buffalo-specific LIF may enhance the efficiency of buESCs by improving

SUBMITTER: Jamwal S 

PROVIDER: S-EPMC8927517 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature