Capturing 3D Chromatin Maps of Human Primary Monocytes: Insights From High-Resolution Hi-C.

ABSTRACT: Although the variation in chromatin architecture during adaptive immune responses has been thoroughly investigated, the 3D landscape of innate immunity is still unknown. Herein, chromatin regulation and heterogeneity among human primary monocytes were investigated. Peripheral blood was collected from two healthy persons and two patients with systemic lupus erythematosus (SLE), and CD14⁺ monocytes were selected to perform Hi-C, RNA-seq, ATAC-seq and ChIP-seq analyses. Raw data from the THP1 cell line Hi-C library were used for comparison. For each sample, we constructed three Hi-C libraries and obtained approximately 3 billion paired-end reads in total. Resolution analysis showed that more than 80% of bins presented depths greater than 1000 at a 5 kb resolution. The constructed h

SUBMITTER: Xia Y 

PROVIDER: S-EPMC8927851 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature