Completing the family of human Eps15 homology domains: Solution structure of the internal Eps15 homology domain of γ-synergin.

ABSTRACT: Eps15 homology (EH) domains are universal interaction domains to establish networks of protein-protein interactions in the cell. These networks mainly coordinate cellular functions including endocytosis, actin remodeling, and other intracellular signaling pathways. They are well characterized in structural terms, except for the internal EH domain from human γ-synergin (EHγ). Here, we complete the family of EH domain structures by determining the solution structure of the EHγ domain. The structural ensemble follows the canonical EH domain fold and the identified binding site is similar to other known EH domains. But EHγ differs significantly in the N- and C-terminal regions. The N-terminal α-helix is shortened compared to known homologues, while the C-terminal one is fully formed. A signifi

SUBMITTER: Kovermann M 

PROVIDER: S-EPMC8927860 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature