Dataset Information

Prevalence and genetic diversity of Enterocytozoon bieneusi in nonhuman primates in Northern and Central China.

ABSTRACT:

Background

Enterocytozoon bieneusi is a common enteric pathogen reported in human and many animals. But there are few reports of E. bieneusi in nonhuman primates (NHPs). The aim of this study was to examine the prevalence and molecular characterization of E. bieneusi in NHPs from Northern and Central China.

Results

A total of 299 specimens of NHPs were collected. The overall prevalence rate of E. bieneusi was 9.4% (28/299) in NHPs by ribosomal internal transcribed spacer (ITS) amplification, including 10.0% (16/160) in captive NHPs and 8.6% (12/139) in wild NHPs. In captive NHPs, the infection rate was 9.1% in male, 11.5% in female. Infection rate in juvenile ITS (6.7%) was higher than in the adult ITS (5.6%). In different regions, infection rate in Hubei (14.7%) was higher than in Henan (7.6%) and Beijing (7.9%). Five genotypes were found, including 4 known genotypes (D, HND-Ⅰ, EbpC, SHW2) and a novel genotype named NHP1. Genotype D (8/28) and NHP1 (8/28) were the most prevalent, followed by EbpC (6/28), SHW2 (4/28), and HND-Ⅰ (2/28). All the 5 genotypes belonged to zoonotic Group1.

Conclusion

These findings could deepen our understanding of E. bieneusi prevalence and genotype distribution in NHPs in China. Our study shows that NHPs may be the reservoir of zoonotic E. bieneusi and might present a potential serious threat.

SUBMITTER: Zhang P

PROVIDER: S-EPMC8927925 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Publications

Prevalence and genetic diversity of <i>Enterocytozoon bieneusi</i> in nonhuman primates in Northern and Central China.

Zhang Peiyang P Feng Shengyong S Jia Ting T Han Shuyi S Zhang Chenglin C He Hongxuan H

International journal for parasitology. Parasites and wildlife 20220215

<h4>Background</h4><i>Enterocytozoon bieneusi</i> is a common enteric pathogen reported in human and many animals. But there are few reports of <i>E. bieneusi</i> in nonhuman primates (NHPs). The aim of this study was to examine the prevalence and molecular characterization of <i>E. bieneusi</i> in NHPs from Northern and Central China.<h4>Results</h4>A total of 299 specimens of NHPs were collected. The overall prevalence rate of <i>E. bieneusi</i> was 9.4% (28/299) in NHPs by ribosomal internal ...[more]

PMID: 35309041

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Project description:BackgroundRodents, globally overpopulated, are an important source for zoonotic disease transmission to humans, including Enterocytozoon bieneusi (one of the most prevalent zoonotic pathogens). Here, we studied the prevalence and performed genetic analyses of E. bieneusi in rodents from the Hainan Province of China.MethodsA total of 603 fresh fecal samples were gathered from 369 wild rats, 117 bamboo rats, 93 Asiatic brush-tailed porcupine and 24 red-bellied squirrels. The wild rats were identified to the species level by amplification of a 421-bp region of the cytb gene from fecal DNA using PCR. Genotype analysis was performed by amplification of the internal transcribed spacer (ITS) region of rDNA of E. bieneusi using PCR.ResultsSeven wild rat species were identified. The average rate of infection with E. bieneusi was 15.8% (95/603) with 18.7% (69/369) in wild rats, 11.9% (25/210) in farmed rodents and 4.2% (1/24) in red-bellied squirrels. Sixteen E. bieneusi genotypes were identified, including 9 known genotypes (D, Type IV, PigEBITS7, Peru8, Peru11, ESH02, S7, EbpA and CHG5), and 7 novel genotypes (HNR-I to HNR-VII). Genotype D (44.2%, 42/95) predominated, followed by PigEBITS7 (20.0%, 19/95), HNR-VII (15.8%, 15/95), Type IV (5.3%, 5/95), HNR-III (2.1%, 2/95), HNR-VI (2.1%, 2/95) and each of the remaining 10 genotypes (1.1%, 1/95). The phylogenetic analysis of the ITS region of E. bieneusi divided the identified genotypes into the following four groups: Group 1 (n = 13), Group 2 (n = 1), Group 12 (n = 1), and the novel Group 13 (n = 1).ConclusionsTo our knowledge, this is the first report on the identification of E. bieneusi in rodents from Hainan, China. The zoonotic potential of the identified E. bieneusi genotypes suggested that the rodents poses a serious threat to the local inhabitants. Thus, measures need to be taken to control the population of wild rats in the areas investigated in this study, along with identification of safe methods for disposal of farmed rodent feces. Additionally, the local people should be made aware of the risk of disease transmission from rodents to humans.