Effects of lipopolysaccharide on follicular estrogen production and developmental competence in bovine oocytes.

ABSTRACT: Reproductive efficiency and female fertility is essential for productive and sustainable beef cattle operations. Gram-negative bacterial infections cause release of the endotoxin lipopolysaccharide (LPS) which initiates immune responses shown to alter ovarian steroidogenesis and impair oocyte development. The current study was designed to investigate the impact of varying levels of naturally occurring infection and follicular LPS on estradiol (E₂) production and oocyte maturation. Bovine ovary pairs were harvested from a slaughterhouse, and oocytes were aspirated from small follicles and matured in vitro. Meiotic events were evaluated on nuclear maturation and spindle morphology to classify oocytes as normal or abnormal. Follicular fluid LPS concentrations were measured and subs

SUBMITTER: Forrest KK 

PROVIDER: S-EPMC8928215 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature