Selective Separation of Highly Similar Proteins on Ionic Liquid-Loaded Mesoporous TiO₂.

ABSTRACT: Separating proteins from their mixtures is an important process in a great variety of applications, but it faces difficult challenges as soon as the proteins are simultaneously of similar sizes and carry comparable net charges. To develop both efficient and sustainable strategies for the selective separation of similar proteins and to understand the underlying molecular mechanisms to enable the separation are crucial. In this work, we propose a novel strategy where the cholinium-based amino acid [Cho][Pro] ionic liquid (IL) is used as the trace additive and loaded physically on a mesoporous TiO₂ surface for separating two similar proteins (lysozyme and cytochrome c). The observed selective adsorption behavior is explained by the hydration properties of the [Cho][Pro] load

SUBMITTER: Dong Y 

PROVIDER: S-EPMC8928471 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature