Gene signature associated with resistance to fluvastatin chemoprevention for breast cancer.

ABSTRACT:

Background

Although targeting of the cholesterol pathway by statins prevents breast cancer development in mouse models, efficacy is not absolute. Therefore, the goal of this study is to investigate if the upregulation in the cholesterol biosynthesis pathway genes associates with response to statin chemoprevention and may potentially be used as response biomarkers.

Methods

Expression of cholesterol biosynthesis pathway genes was initially derived from the RNA sequencing of MCF10A cell line- based breast cancer progression model system and subsequently validated by quantitative PCR assay. Response to fluvastatin was assessed in vitro using the MCF10A cell line model system, including a statin resistant cell line that was generated (MCF10.AT1-R), and measured using colony formi

SUBMITTER: Bhardwaj A 

PROVIDER: S-EPMC8928625 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature