Insights regarding sirtuin-dependent gene regulation during white koji production.

ABSTRACT: White koji, a solid-state culture of Aspergillus luchuensis mut. kawachii using grains such as rice and barley, is used as a source of amylolytic enzymes and citric acid for the production of shochu, a traditional Japanese distilled spirit. We previously characterized changes in gene expression that affect the properties of white koji during the shochu production process; however, the underlying regulatory mechanisms were not determined. We then characterized the NAD⁺-dependent histone deacetylase sirtuin, an epigenetic regulator of various biological phenomena, in A. l. mut. kawachii and found that sirtuin SirD is involved in expression of α-amylase activity and citric acid accumulation. In this addendum study, we measured the NAD⁺/NADH re

SUBMITTER: Futagami T 

PROVIDER: S-EPMC8928858 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature