Hemopexin Modulates Expression of Complement Regulatory Proteins in Rat Glomeruli.

ABSTRACT: In systemic hemolysis and in hematuric forms of kidney injury, the major heme scavenging protein, hemopexin (HPX), becomes depleted, and the glomerular microvasculature (glomeruli) is exposed to high concentrations of unbound heme, which, in addition to causing oxidative injury, can activate complement cascades; thus, compounding extent of injury. It is unknown whether unbound heme can also activate specific complement regulatory proteins that could defend against complement-dependent injury. Isolated rat glomeruli were incubated in media supplemented with HPX-deficient (HPX^-) or HPX-containing (HPX⁺) sera as a means of achieving different degrees of heme partitioning between incubation media and glomerular cells. Expression of heme oxygenase (HO)-1 and of the complem

SUBMITTER: Detsika MG 

PROVIDER: S-EPMC8928991 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature