The airway microbiota of non-small cell lung cancer patients and its relationship to tumor stage and EGFR gene mutation.

ABSTRACT:

Background

Accumulating studies have suggested the airway microbiota in lung cancer patients is significantly different from that of healthy controls. However, little is known about the relationship between airway microbiota and important clinical parameters of lung cancer. In this study, we aimed to explore the association between sputum microbiota and lung cancer stage, lymph node metastasis, intrathoracic metastasis, and epidermal growth factor receptor (EGFR) gene mutation.

Methods

The microbiota of sputum samples from 85 newly-diagnosed NSCLC patients were sequenced via 16S rRNA sequencing of the V3-V4 region. Sequencing reads were filtered using QIIME2 and clustered against UPARSE.

Results

Alpha- and β-diversity was significantly different between patients in st

SUBMITTER: Huang DH 

PROVIDER: S-EPMC8930493 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature