Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cells.

ABSTRACT: Proper segregation of chromosomes during mitosis depends on "amphitelic attachments"-load-bearing connections of sister kinetochores to the opposite spindle poles via bundles of microtubules, termed as the "K-fibers." Current models of spindle assembly assume that K-fibers arise largely from stochastic capture of microtubules, which occurs at random times and locations and independently at sister kinetochores. We test this assumption by following the movements of all kinetochores in human cells and determine that most amphitelic attachments form synchronously at a specific stage of spindle assembly and within a spatially distinct domain. This biorientation domain is enriched in bundles of antiparallel microtubules, and perturbation of microtubule bundling changes the temporal and spatial d

SUBMITTER: Renda F 

PROVIDER: S-EPMC8930511 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature