Evidence that the transcriptional repressor ICER is regulated via the N-end rule for ubiquitination.

ABSTRACT: ICER is a transcriptional repressor that is mono- or poly-ubiquitinated. This either causes ICER to be translocated from the nucleus, or degraded via the proteasome, respectively. In order to further studies the proteins involved in ICER regulation mass spectrometry analysis was performed to identify potential candidates. We identified twenty eight ICER-interacting proteins in human melanoma cells, Sk-Mel-24. In this study we focus on two proteins with potential roles in ICER proteasomal degradation in response to the N-end rule for ubiquitination: the N-alpha-acetyltransferase 15 (NAA15) and the E3 ubiquitin-protein ligase UBR4. Using an HA-tag on the N- or C-terminus of ICER (_NHAICER or ICER_CHA) it was found that the N-terminus of ICER is important for its interacti

SUBMITTER: Cirinelli A 

PROVIDER: S-EPMC8930515 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature