Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules.

ABSTRACT: Chromosome segregation is accomplished by the mitotic spindle, a bipolar micromachine built primarily from microtubules. Different microtubule populations contribute to spindle function: kinetochore microtubules attach and transmit forces to chromosomes, antiparallel interpolar microtubules support spindle structure, and astral microtubules connect spindle poles to the cell cortex.^1,2 In mammalian cells, end-binding (EB) proteins associate with all growing microtubule plus ends throughout the cell cycle and serve as adaptors for diverse +TIPs that control microtubule dynamics and interactions with other intracellular structures.³ Because binding of many +TIPs to EB1 and thus microtubule-end association is switched off by mitotic phosphorylation,<

SUBMITTER: Dema A 

PROVIDER: S-EPMC8930524 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature