A tiered approach to population-based in vitro testing for cardiotoxicity: Balancing estimates of potency and variability.

ABSTRACT: Population-wide in vitro studies for characterization of cardiotoxicity hazard, risk, and population variability show that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are a powerful and high-throughput testing platform for drugs and environmental chemicals alike. However, studies in multiple donor-derived hiPSC-CMs, across large libraries of chemicals tested in concentration-response are technically complex, and study design optimization is needed to determine sufficient and fit-for-purpose population size considerations. Therefore, we tested a hypothesis that a computational down-sampling analysis based on the data from hiPSC-CM screening of 136 diverse compounds in a population of 43 non-diseased donors, including multiple replicates of the "standard" donor hiPS

SUBMITTER: Blanchette AD 

PROVIDER: S-EPMC8930538 | biostudies-literature | 2022 Mar-Apr

REPOSITORIES: biostudies-literature