Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiation.

ABSTRACT: Translational control shapes the proteome in normal and pathophysiological conditions. Current high-throughput approaches reveal large differences in mRNA-specific translation activity but cannot identify the causative mRNA features. We developed direct analysis of ribosome targeting (DART) and used it to dissect regulatory elements within 5' untranslated regions that confer 1,000-fold differences in ribosome recruitment in biochemically accessible cell lysates. Using DART, we determined a functional role for most alternative 5' UTR isoforms expressed in yeast, revealed a general mode of increased translation via direct binding to a core translation factor, and identified numerous translational control elements including C-rich silencers that are sufficient to repress translation both in v

SUBMITTER: Niederer RO 

PROVIDER: S-EPMC8930539 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature