Differential Kinase Activity Across Prostate Tumor Compartments Defines Sensitivity to Target Inhibition.

ABSTRACT: Cancer therapy often results in heterogeneous responses in different metastatic lesions in the same patient. Inter- and intratumor heterogeneity in signaling within various tumor compartments and its impact on therapy are not well characterized due to the limited sensitivity of single-cell proteomic approaches. To overcome this barrier, we applied single-cell mass cytometry with a customized 26-antibody panel to PTEN-deleted orthotopic prostate cancer xenograft models to measure the evolution of kinase activities in different tumor compartments during metastasis or drug treatment. Compared with primary tumors and circulating tumor cells (CTC), bone metastases, but not lung and liver metastases, exhibited elevated PI3K/mTOR signaling and overexpressed receptor tyrosine kinases (RTK) includi

SUBMITTER: Karabacak NM 

PROVIDER: S-EPMC8930560 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature