Unconcerted conformational changes in Arp2/3 complex integrate multiple activating signals to assemble functional actin networks.

ABSTRACT: Arp2/3 complex nucleates branched actin filaments important for processes such as DNA repair, endocytosis, and cellular motility. Multiple factors are required to activate branching nucleation by Arp2/3 complex, including a WASP family protein and a pre-existing actin filament. Activation is achieved through two major conformational changes-subunit flattening and movement into the short pitch conformation-that allow the actin-related proteins (Arps) within the complex (Arp2 and Arp3) to mimic filamentous actin subunits, thereby templating new filament assembly. Some models suggest that these changes are concerted and stimulated cooperatively by WASP and actin filaments, but how Arp2/3 complex integrates signals from multiple factors to drive switch-like activation of branching nucleation h

SUBMITTER: Narvaez-Ortiz HY 

PROVIDER: S-EPMC8930562 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature