Impact of Early Feeding: Metagenomics Analysis of the Infant Gut Microbiome.

ABSTRACT:

Background

Different feeding regimens in infancy alter the gastrointestinal (gut) microbial environment. The fecal microbiota in turn influences gastrointestinal homeostasis including metabolism, immune function, and extra-/intra-intestinal signaling. Advances in next generation sequencing (NGS) have enhanced our ability to study the gut microbiome of breast-fed (BF) and formula-fed (FF) infants with a data-driven hypothesis approach.

Methods

Next generation sequencing libraries were constructed from fecal samples of BF (n=24) and FF (n=10) infants and sequenced on an Illumina HiSeq 2500. Taxonomic classification of the NGS data was performed using the Sunbeam/Kraken pipeline and a functional analysis at the gene level was performed using publicly available algorithms, inclu

SUBMITTER: Di Guglielmo MD 

PROVIDER: S-EPMC8931315 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature