Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing.

ABSTRACT: Background: Automation has increasingly become more commonplace in the research laboratory workspace. The introduction of articulated robotic arms allows the researcher more flexibility in the tasks a single piece of automated machinery can perform. We set out to incorporate automation in processing of genomic DNA organic extractions to increase throughput and limit researchers to the exposure of organic solvents. Methods: In order to automate the genome sequencing pipeline in our laboratory, we programmed a dual-arm anthropomorphic robot, the Robotic Biology Institute's Maholo LabDroid, to perform organic solvent-based genomic DNA extraction from cell lysates. To the best of our knowledge, this is the first time that automation of phenol-chloroform extraction has been report

SUBMITTER: Liu AW 

PROVIDER: S-EPMC8931447 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature