Quantitative Imaging of Bacteriophage Amplification for Rapid Detection of Bacteria in Model Foods.

ABSTRACT: Rapid detection of bacteria in water and food samples is a critical need. The current molecular methods like real-time PCR can provide rapid detection after initial enrichment. However, these methods require significant preparation steps, specialized facilities to reduce contamination, and relatively expensive reagents. This study evaluates a novel approach for detecting bacteria based on imaging of bacteriophage amplification upon infection of the target host bacteria to mitigate some of these constraints and improve the specificity of discriminating live vs. dead bacteria. Thus, this research leverages the natural ability of lytic bacteriophages to rapidly amplify their genetic material and generate progeny phages upon infecting the host bacterium. This study uses a nucleic acid staining

SUBMITTER: Wisuthiphaet N 

PROVIDER: S-EPMC8931685 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature