A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants.

ABSTRACT:

Background

The genetic engineering of crops has enhanced productivity in the face of climate change and a growing global population by conferring desirable genetic traits, including the enhancement of biotic and abiotic stress tolerance, to improve agriculture. The clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system has been found to be a promising technology for genomic editing. Protoplasts are often utilized for the development of genetically modified plants through in vitro integration of a recombinant DNA fragment into the plant genome. We targeted the citrus Nonexpressor of Pathogenesis-Related 3 (CsNPR3) gene, a negative regulator of systemic acquired resistance (SAR) that governs the proteasome-mediated degradation of NPR1 and developed a genome ed

SUBMITTER: Mahmoud LM 

PROVIDER: S-EPMC8932238 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature