A touch transfer assay to determine surface transmission of highly pathogenic viruses.
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ABSTRACT:
SUBMITTER: Meister TL
PROVIDER: S-EPMC8932683 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
A touch transfer assay to determine surface transmission of highly pathogenic viruses.
Meister Toni Luise TL Brüggemann Yannick Y Tamele Barbora B Howes John J Steinmann Eike E Todt Daniel D
STAR protocols 20220318 2
Transmission via fomites poses a major dissemination route for many human pathogens, particularly because of transfer via fingertips. Here, we present a protocol to investigate direct transfer of infectious agents from fomites to humans via naked fingertips. The protocol is suitable for pathogens requiring highest biosafety levels (e.g., SARS-CoV-2). We used an artificial skin to touch a defined volume of virus suspension and subsequent quantification of infectious entities allows quantitative m ...[more]
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