LncSNHG1 Promoted CRC Proliferation through the miR-181b-5p/SMAD2 Axis.

ABSTRACT:

Objective

To investigate the effects of LncRNA SNHG1 on the proliferation, migration, and epithelial-mesenchymal transition (EMT) of colorectal cancer cells (CRCs).

Methods

4 pairs of CRC tissue samples and their corresponding adjacent samples were analyzed by the human LncRNA microarray chip. The expression of LncSNHG1 in CRC cell lines was verified by qRT-PCR. Colony formation assays and CCK8 assays were applied to study the changes in cell proliferation. The transwell assay and wound healing experiments were used to verify the cell invasion and migration. EMT progression was confirmed finally.

Results

LncSNHG1 was overexpressed both in CRC tissues and cell lines, while the miR-181b-5p expression was decreased in CRC cell lines. After knock-down of LncSNHG1, the pro

SUBMITTER: Huang Q 

PROVIDER: S-EPMC8933095 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature