Neurofunctional and neuroimaging readouts for designing a preclinical stem-cell therapy trial in experimental stroke.

ABSTRACT: With the aim of designing a preclinical study evaluating an intracerebral cell-based therapy for stroke, an observational study was performed in the rat suture model of ischemic stroke. Objectives were threefold: (i) to characterize neurofunctional and imaging readouts in the first weeks following transient ischemic stroke, according to lesion subtype (hypothalamic, striatal, corticostriatal); (ii) to confirm that intracerebral administration does not negatively impact these readouts; and (iii) to calculate sample sizes for a future therapeutic trial using these readouts as endpoints. Our results suggested that the most relevant endpoints were side bias (staircase test) and axial diffusivity (AD) (diffusion tensor imaging). Hypothalamic-only lesions did not affect those parameters, which w

SUBMITTER: Dumot C 

PROVIDER: S-EPMC8933390 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature