DNA-based platform for efficient and precisely targeted bioorthogonal catalysis in living systems.

ABSTRACT: As one of the typical bioorthogonal reactions, copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction holds great potential in organic synthesis, bioconjugation, and surface functionalization. However, the toxicity of Cu(I), inefficient catalytic activity, and the lack of cell specific targeting of the existing catalysts hampered their practical applications in living systems. Herein, we design and construct a DNA-based platform as a biocompatible, highly efficient, and precisely targeted bioorthogonal nanocatalyst. The nanocatalyst presents excellent catalytic efficiency in vitro, which is one order of magnitude higher than the commonly used catalyst CuSO₄/sodium ascorbate. The theoretical calculation further supports the contribution of DNA structure and its interacti

SUBMITTER: You Y 

PROVIDER: S-EPMC8933418 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature