Analysis tools for single-monomer measurements of self-assembly processes.

ABSTRACT: Protein assembly plays an important role throughout all phyla of life, both physiologically and pathologically. In particular, aggregation and polymerization of proteins are key-strategies that regulate cellular function. In recent years, methods to experimentally study the assembly process on a single-molecule level have been developed. This progress concomitantly has triggered the question of how to analyze this type of single-filament data adequately and what experimental conditions are necessary to allow a meaningful interpretation of the analysis. Here, we developed two analysis methods for single-filament data: the visitation analysis and the average-rate analysis. We benchmarked and compared both approaches with the classic dwell-time-analysis frequently used to study microscopic as

SUBMITTER: Hoyer M 

PROVIDER: S-EPMC8933434 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature