Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion.

ABSTRACT: Glucuronoyl esterases (GEs) are α/β serine hydrolases and a relatively new addition in the toolbox to reduce the recalcitrance of lignocellulose, the biggest obstacle in cost-effective utilization of this important renewable resource. While biochemical and structural characterization of GEs have progressed greatly recently, there have yet been no mechanistic studies shedding light onto the rate-limiting steps relevant for biomass conversion. The bacterial GE OtCE15A possesses a classical yet distinctive catalytic machinery, with easily identifiable catalytic Ser/His completed by two acidic residues (Glu and Asp) rather than one as in the classical triad, and an Arg side chain participating in the oxyanion hole. By QM/MM calculations, we identified deacylation as the decisive step in cataly

SUBMITTER: Zong Z 

PROVIDER: S-EPMC8933493 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature