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Optimization of an O₂-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.

ABSTRACT: A bioartificial pancreas (BAP) encapsulating high pancreatic islets concentration is a promising alternative for type 1 diabetes therapy. However, the main limitation of this approach is O₂ supply, especially until graft neovascularization. Here, we described a methodology to design an optimal O₂-balanced BAP using statistical design of experiment (DoE). A full factorial DoE was first performed to screen two O₂-technologies on their ability to preserve pseudo-islet viability and function under hypoxia and normoxia. Then, response surface methodology was used to define the optimal O₂-carrier and islet seeding concentrations to maximize the number of viable pseudo-islets in the BAP containing an O₂-generator under hypoxia. Monitoring of viability, function and maturation of neonatal pig islets for 15 days in vitro demonstrated the efficiency of the optimal O₂-balanced BAP. The findings should allow the design of a more realistic BAP for humans with high islets concentration by maintaining the O₂ balance in the device.

SUBMITTER: Moure A

PROVIDER: S-EPMC8933496 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.

Mouré Anne A Bekir Sawsen S Bacou Elodie E Pruvost Quentin Q Haurogné Karine K Allard Marie M De Beaurepaire Laurence L Bosch Steffi S Riochet David D Gauthier Olivier O Blancho Gilles G Soulillou Jean-Paul JP Poncelet Denis D Mignot Grégoire G Courcoux Philippe P Jegou Dominique D Bach Jean-Marie JM Mosser Mathilde M

Scientific reports 20220318 1

A bioartificial pancreas (BAP) encapsulating high pancreatic islets concentration is a promising alternative for type 1 diabetes therapy. However, the main limitation of this approach is O2 supply, especially until graft neovascularization. Here, we described a methodology to design an optimal O2-balanced BAP using statistical design of experiment (DoE). A full factorial DoE was first performed to screen two O2-technologies on their ability to preserve pseudo-isl ...[more]

PMID: 35304495

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Project description:Aiming at the problems of low utilization rate of corn fertilizer, low precision of fertilization ratio, and time-consuming and laborious topdressing in the later stage, an U-shaped fertilization device with uniform fertilizer mechanism was designed. The device was mainly composed of uniform fertilizer mixing mechanism, fertilizer guide plate and fertilization plate. Compound fertilizer was applied on both sides and slow/controlled release fertilizer was applied at the bottom to form an U-shaped distribution of fertilizer around corn seeds. Through theoretical analysis and calculation, the structural parameters of the fertilization device were determined. Through the simulated soil tank test, the quadratic regression orthogonal rotation combination design was carried out on the main factors affecting the spatial stratification effect of fertilizer. The optimal parameters were obtained as follows: the stirring speed of the stirring structure was 300 r/min, the bending angle of the fertilization tube was 165°, and the operating speed of the fertilization device was 3 km/h. The results of bench verification test showed that under the optimized stirring speed and bending angle, the fertilizer particles were stirred evenly, and the average values of fertilizer flowing out of the fertilization tubes on both sides were 299.5 g and 297.4 g, respectively. The average fertilizer amounts of the three fertilizer outlets were 200.4 g, 203.2 g and 197.7 g, respectively, which met the agronomic requirements of 1:1:1 fertilization, and the variation coefficients of fertilizer amounts on both sides of the fertilizer pipe and each layer were less than 0.1% and 0.4%, respectively. The simulation results of the optimized U-shaped fertilization device can achieve the expected U-shaped fertilization effect around corn seeds. The results of field experiment showed that the U-shaped fertilization device could realize the U-shaped proportional application of fertilizer in soil. The distance between the upper end of fertilization on both sides and the distance between the base fertilizer and the surface were 87.3-95.2 mm and 197.8-206.0 mm, respectively. The transverse distance between the fertilizers on both sides was 84.3-99.4 mm, and the error with the designed theoretical fertilization was within 10 mm. Compared with the traditional side fertilization method, the number of corn roots increased by 5-6, the root length increased by 30-40 mm, and the yield increased by 9.9-14.8%.

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Optimization of an O₂-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.

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Optimization of an O<sub>2</sub>-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.

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