Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling.

ABSTRACT: Layers of genome organization are becoming increasingly better characterized, but less is known about how these structures respond to perturbation or shape changes. Low-salt swelling of isolated chromatin fibers or nuclei has been used for decades to investigate the structural properties of chromatin. But, visible changes in chromatin appearance have not been linked to known building blocks of genome structure or features along the genome sequence. We combine low-salt swelling of isolated nuclei with genome-wide chromosome conformation capture (Hi-C) and imaging approaches to probe the effects of chromatin extension genome-wide. Photoconverted patterns on nuclei during expansion and contraction indicate that global genome structure is preserved after dramatic nuclear volume swelling, sugge

SUBMITTER: Sanders JT 

PROVIDER: S-EPMC8933507 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature