Synthetic exposure with a CMOS camera for multiple exposure speckle imaging of blood flow.

ABSTRACT: Speckle contrast imaging is an established technique to obtain relative blood flow maps over wide fields of view. A major improvement of the method relies on the acquisition of raw speckle images at different exposure times but requires simultaneous modulation of a laser pulse in duration and intensity and precise synchronization with a camera. This complex instrumentation has limited the use of multiple exposure speckle imaging. We evaluate here the use of a CMOS camera for a simplified approach based on synthetic exposure images created from the sum of successive frames acquired at a 1 ms exposure time. Both methods have been applied to evaluate controlled flows in micro-channels. The contribution of noises to the speckle contrast have been quantified and compared. Dark, readout and shot

SUBMITTER: Chammas M 

PROVIDER: S-EPMC8933569 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature