Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system.

ABSTRACT:

Background

Lipoxygenase (LOX) is a non-heme iron containing dioxygenase that is widely used to improve food quality and produce active drug intermediates and biodiesel. Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression; however, its weak extracellular secretion ability precludes its effective production of recombinant proteins into the extracellular environment. To facilitate subsequent characterization and application of LOX, improving its secretion efficiency from E. coli is a major challenge that needs to be solved.

Results

Several strategies were adopted to improve the extracellular secretion of LOX based on the signal peptides and cell wall permeability of E. coli. Here, we studied the effect of signal peptides on LOX

SUBMITTER: Pang C 

PROVIDER: S-EPMC8933919 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature