Parecoxib inhibits esophageal squamous cell carcinoma progression via the PDK1-AKT pathway.

ABSTRACT:

Background

Parecoxib plays an important role in inhibition of human cancer. However, the effect of parecoxib on esophageal squamous cell carcinoma (ESCC) is still not well known. The purpose of this study was to investigate the effect of parecoxib on ESCC and its underlying mechanism.

Methods

RNA-sequence analysis was performed to identify functional alterations and mechanisms. Cell cycle, proliferation, invasion, and migration were assessed using flow cytometry, CCK-8 assay, colony formation, transwell, and wound healing assays. Extracellular matrix (ECM) degradation was detected by substrate gel zymography and 3D cell culture assay. Western blotting was used to detect parecoxib-dependent mechanisms involving cell cycle, proliferation, invasion, and migration. Tumor formati

SUBMITTER: Huang HM 

PROVIDER: S-EPMC8933956 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature