Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia-Laos border.

ABSTRACT:

Background

To develop an effective malaria vector intervention method in forested international border regions within the Greater Mekong Subregion (GMS), more in-depth studies should be conducted on local Anopheles species composition and bionomic features. There is a paucity of comprehensive surveys of biodiversity integrating morphological and molecular species identification conducted within the border of Laos and Cambodia.

Methods

A total of 2394 adult mosquitoes were trapped in the Cambodia-Laos border region. We first performed morphological identification of Anopheles mosquitoes and subsequently performed molecular identification using 412 recombinant DNA-internal transcribed spacer 2 (rDNA-ITS2) and 391 mitochondrial DNA-cytochrome c oxidase subunit 2 (mtDNA-COII) se

SUBMITTER: Zhang C 

PROVIDER: S-EPMC8933986 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature