Mesenchymal Stem Cell-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression.

ABSTRACT:

Background

Regulatory T cell (Treg)/T helper (Th) 17 skewing is important in the development of acute respiratory distress syndrome (ARDS). Immunomodulatory effects of mesenchymal stem cell- (MSC-) secreted transforming growth factor- (TGF-) β1 on CD4⁺ T cells are environment-sensitive and lack discussion in hypoxic and inflammatory conditions.

Methods

Mouse splenic CD4⁺ T cells were precoated with anti-CD3 (5 μg/ml) and anti-CD28 (2 μg/ml) overnight. RAW264.7 cells were added as antigen-presenting cells (APCs). T cells with and without RAW264.7 cells were treated with various LPS concentrations of 0, 10, 100, and 1000 ng/ml or/and at hypoxia condition of 5% O₂. Based on LPS (100 ng/ml) and hypoxia conditions (5% O_2<

SUBMITTER: Xue M 

PROVIDER: S-EPMC8934213 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature