Identification of novel alternative splicing associated with mastitis disease in Holstein dairy cows using large gap read mapping.

ABSTRACT:

Background

Mastitis is a very common disease in the dairy industry that producers encounter daily. Transcriptomics, using RNA-Sequencing (RNA - Seq) technology, can be used to study the functional aspect of mastitis resistance to identify animals that have a better immune response to mastitis. When the cow has mastitis, not only genes but also specific mRNA isoforms generated via alternative splicing (AS) could be differentially expressed (DE), leading to the phenotypic variation observed. Therefore, the objective of this study was to use large gap read mapping to identify mRNA isoforms DE between healthy and mastitic milk somatic cell samples (N = 12). These mRNA isoforms were then categorized based on being 1) annotated mRNA isoforms for gene name and length, 2) annotated mRNA is

SUBMITTER: Asselstine V 

PROVIDER: S-EPMC8934477 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature