Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo.

ABSTRACT:

Background

Silica nanoparticles (SiO₂ NPs) are extensively applied in the biomedical field. The increasing medical application of SiO₂ NPs has raised concerns about their safety. However, studies on SiO₂ NP-induced retinal toxicity are lacking.

Methods

We investigated the retinal toxicity of SiO₂ NPs with different sizes (15 and 50 nm) in vitro and in vivo along with the underlying mechanisms. The cytotoxicity of SiO₂ NPs with different sizes was assessed in R28 human retinal precursor cells by determining the ATP content and LDH release. The cell morphologies and nanoparticle distributions in the cells were analyzed by phase-contrast microscopy and transmission electron microscopy, respectively. The mitochondrial membra

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8934510 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature